Dana-Farber Cancer Institute: Mapping the Function of Rare Oncogenic Variants

Although some oncogenes and tumor suppressor genes are recurrently mutated at high frequency, the majority of somatic sequence alterations found in cancers occur at low frequency, and the functional consequences of the majority of these mutated alleles remain unknown. We are developing a scalable systematic approach to interrogate the function of cancer-associated gene variants.

Read the abstract: Kim et al., 2016

Experimental Approaches

We have developed an approach to generate mutant forms of cDNA clones at high efficiency and are introducing these into cell models to determine their impact on gene expression and tumorigenesis.

Read the detailed Experimental Approaches: Kim et al., 2016

If you cannot access the manuscript, or if you have additional questions, please email Joshua Dempster.

Data

Access the CTD2 Data Portal.

 

Last updated: April 20, 2017