Cooperative blockade of PKCα and JAK2 drives apoptosis in glioblastoma.

Brain Cancer Regions

Brain Cancer Regions (CC BY-SA 4.0)

Wong RA, Luo X, Lu M, An Z, Haas-Kogan DA, Philips JJ, Shokat KM, Weiss WA, Fan QW.

Cancer Research

December 05, 2019

The mechanistic target of rapamycin (mTOR) signaling is dysregulated prominently in human cancers including glioblastoma, suggesting mTOR as a robust target for therapy. Inhibitors of mTOR have had limited success clinically however, in part because their mechanism of action is cytostatic rather than cytotoxic. Here, we tested three distinct mTOR kinase inhibitors (TORKis) PP242, KU-0063794, and sapanisertib against glioblastoma cells. All agents similarly decreased proliferation of glioblastoma cells, whereas PP242 uniquely induced apoptosis. Apoptosis induced by PP242 resulted from off-target cooperative inhibition of Janus kinase 2 (JAK2) and protein kinase C alpha (PKCα). Induction of apoptosis was also decreased by additional on-target inhibition of mTOR, due to induction of autophagy. As EGFR inhibitors can block PKCα, EGFR inhibitors erlotinib and osimertinib were tested separately in combination with the JAK2 inhibitor AZD1480. Combination therapy induced apoptosis of glioblastoma tumors in both flank and in patient-derived orthotopic xenograft models, providing a preclinical rationale to test analogous combinations in patients.

Program:
CTD²
Last updated: December 20, 2019