Candidate Cancer Allele cDNA Collection

CTD2 researchers at the Broad Institute/DFCI have developed a collection of plasmids including mutant alleles found in sequencing studies of cancer. It includes somatic variants found in lung adenocarcinoma and across other cancer types. The clones enable researchers to characterize the function of the cancer variants in a high throughput experiments. These plasmids are collectively called the “Broad Target Accelerator Plasmid Collections”. The design and construction of these plasmids is described in the manuscripts listed below and are available through a distributor: https://www.addgene.org/kits/boehm-target-accelerator-cancer-collection/

References

Primary

Kim E, et al. Systematic functional interrogation of rare cancer variants identifies oncogenic alleles. Cancer Discovery. 2016 Jul;6(7):714-26. (PMID: 27147599)

Berger AH, Brooks AN, Wu X, et al. High-throughput phenotyping of lung cancer somatic mutations. Cancer Cell. 2016 Aug 8;30(2):214-28. (PMID: 27478040)
 
Supporting

Rohban M, et al. Systematic morphological profiling of human gene and allele function via Cell Painting. Elife2017 Mar 18;6. pii: e24060. (PMID: 28315521)

Johannessen C, et al. Progress towards precision functional genomics in cancer. Current Opinion in Systems Biology. (2):73-82. Download here

Yang X, et al. A public genome-scale lentiviral expression library of human ORFs. Nature Methods. 2011 Jun 26;8(8):659-61. (PMID: 21706014)
Last updated: December 18, 2018